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[Cell-biology] Request For Help

[Cell-biology] Request For Help - Cell Biology and Cell Culture

[Cell-biology] Request For Help - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.

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Old 09-14-2009, 09:26 PM
Jayakumar, R
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Default [Cell-biology] Request For Help

The paper from bell Labs IS very interesting. But from your answer (fromthe organelles you are interested in), it is clear that you intend to work on eukaryotic cells, but it is still not clear what kind of cells you have in mind, since each eukaryotic cell is a highly specialized "piece of equipment", nor is it clear to what extend you want to take your experiments forward and the expected observation you intend to make. Do you have a hypothesis which you intend to test?? That would help.
As you might know, the immune system is replete with similar memory responses, especially related to plasma cells and long term immunity against diseases. I would suggest researching papers on plasma cell longevity, long term immunity versus short lived immunity and memory T-cells in literature. It has been an enigma how plasma cells in the bone marrow recognize and maintain immunity by producing antibodies against certain pathogenic antigens like that of small pox virus, when they don't do the same for other antigens like the cold virus. A clue might be that the small pox virus is dangerous while the cold virus is not. You should proceed inthat direction with your experiments.. Two variables in your experiments should be TIME (intervals between exposures) and INTENSITY (concentration and number) of the stimulus. An experiment to study such memory responses in cells should consist of repeated exposure of the cell you have inmind to a stressful stimulus(protein, heat, nutrient deprivation or someother factor towards which the particular cell type or types you are interested in are specialized to respond to), varying the time periods of exposure and the dosage of the stimulant. Studying chromosomal translocations.
Please correct me, if I am barking up the wrong tree :-)). Again a hypothetical model which you aim to test, with aims would be useful for people to help you with experiments.
Best ofluck

dddddddddddddddddddddddddddddddddddddddddddddddddd dd
Jayakumar R. Nair, Ph.D.,
HRI Scientist,
Dept. of Immunology,
Roswell Park Cancer Institute,
Elm and Carlton St.
Buffalo, NY 14263
Tel: 716-845-8231
email: [Only registered users see links. ]

"Nil magnum nisi bonum"

-----Original Message-----
From: jitesh dundas [mailto:[Only registered users see links. ]]
Sent: Monday, September 14, 2009 2:54 PM
To: Jayakumar, R
Subject: Re: [Cell-biology] Request For Help

Dear Jay,

Thank you for your reply.

Your question is very vague.
Sorry I don't agree with you. I was trying to keep it brief. In fact, youare the first expert who didn't get my question. I guess every person thinks differently.

you need to be more specific.
Yes, I am provided I get a positive response from an expert like you.
Morever, I am cautious of not providing my research paper details to anyone without authentication.

*What is "thinking capabilities" of cell organelles and what kind of organisms are you planning to study?
WELL, as humans have brain for thinking & control of major operations.I want to verify/investigate if cells can do the same. I maybe looking at the cell/brain analogy besides studying cell behavior.To what level do cells reflect brain-like behavior..

Few papers (one froma bell laba@pubmed titled -can cells think?)

And what are the "tests" or experiments you have in mind? *Please elaborate.

First I just want to study cellular behavior in different conditions likeextreme temperature, pathogen infiltration,etc..

Nucleus is my primary organelle of interest besides mitochondria, edoplasmic reticulum,etc.

I am looking at all possible ways to find if cells can reproduce creativethinking capabilities.

I hope this helps. Please suggest computational tools and wet experimentssuitable for this hypothesis.

Jitesh Dundas

On 9/14/09, Jayakumar, R <[Only registered users see links. ]> wrote:

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