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Protein Concentration Question

Protein Concentration Question - Cell Biology and Cell Culture

Protein Concentration Question - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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  #1  
Old 08-28-2009, 04:52 AM
Mina M Jalali
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Default Protein Concentration Question



Hey Erik,
I saw your question posted on bio.net and was wondering if you ever figured out the answer. I had a similiar question: What is the concentration of a protein in a cell in molarity? I know that proteins account for 15% of a cell's overall concentration and that the molar mass of a protein is 50,000 g/mol. If you could help me figure out the answer, that would be great.

Thank you!
Mina






Hi netters. Can anyone be of help by telling me where/how I might be able
to find/calculate a "typical" concentration (mg/ml or something of the
like) of a housekeeping protein like tubulin in a "typical" mammalian
cell. I need a ball park figure. As far as I know, about 16-20% of a
cell's weight is protein (but what's the typical weight of a "typical"
mammalian cell?), volume is readily estimated from cell diameter, and a
highly expressed protein could be about 1% of the total protein in a cell
(so for, say tubulin, about 0.1-0.2% of the cell is protein by weight
(?)). Any suggestions, info???? Please email.

Cheers,

erik

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  #2  
Old 08-28-2009, 10:45 PM
Bob
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Default Protein Concentration Question

On Thu, 27 Aug 2009 23:52:14 -0500 (CDT), Mina M Jalali
<[Only registered users see links. ]> wrote:


Why not just assume density of cytoplasm is about 1. Close enough for
an estimate of this type. The rest is simple chem.

bob

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  #3  
Old 02-22-2012, 08:09 PM
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Default Re: Protein Concentration Question

thanks
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