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Adult hippocampal progenitor cell culture (AHP)
I am attempting to do an adult hippocampal progenitor cell culture from mice. I am following the general instructions found in papers that established this technique, but I haven't been able to grow the progenitors. After plating I get a variety of cells in my dish, but most of them (round, bright ones) detach and float in the medium. Is there any step-by-step protocol available that would help me troubleshooting? Does somebody have experience with this culture?
Re: Adult hippocampal progenitor cell culture (AHP)
Hi Lucas. This protocol worked great for us.
The Journal of Neuroscience, November 23, 2005, 25(47):10815-10821; doi:10.1523/JNEUROSCI.3249-05.2005
|adult , ahp , cell , culture , hippocampal , progenitor|
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