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Adult hippocampal progenitor cell culture (AHP)

Adult hippocampal progenitor cell culture (AHP) - Cell Biology and Cell Culture

Adult hippocampal progenitor cell culture (AHP) - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 09-29-2009, 09:21 PM
Pipette Filler
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Default Adult hippocampal progenitor cell culture (AHP)



Hi all,
I am attempting to do an adult hippocampal progenitor cell culture from mice. I am following the general instructions found in papers that established this technique, but I haven't been able to grow the progenitors. After plating I get a variety of cells in my dish, but most of them (round, bright ones) detach and float in the medium. Is there any step-by-step protocol available that would help me troubleshooting? Does somebody have experience with this culture?
Thank you!
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Old 10-06-2009, 09:05 PM
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Default Re: Adult hippocampal progenitor cell culture (AHP)

Hi Lucas. This protocol worked great for us.

The Journal of Neuroscience, November 23, 2005, 25(47):10815-10821; doi:10.1523/JNEUROSCI.3249-05.2005

Good luck
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Old 02-22-2012, 07:57 PM
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Default Re: Adult hippocampal progenitor cell culture (AHP)

thanks
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