My PC12 cells frequently got contaminated recently. I usually use 320x invert microscope to look at the cells, and if they are contaminated by conventional bacteria (with cell wall) I can clearly see those "rods". But these days the medium remains clear for 5-6 days and then suddenly turns turbid overnight. I observe the flask under 320x magnification again, but cannot see the usual bacteria. Instead I vaguely see very tiny "-"shaped vibrating things. They are densely present in the culture, while the PC12 cells detach and I suppose they're dead. I suspect if it's mycoplasma contamination? Many websites say they can be hardly detected by conventional microscopy, so I doubt whether what I saw are really mycoplasma... Has anybody actually seen the mycoplasma before?
Thanks in advance for any comments!