I am absolutely desperate right now! I am trying to culture cells from blood from patients with scleroderma but the cells keep dying!
This is my first research position and I have gone through 2 months and the cells keep dying. I am able to isolate the cells fine, but I can't culture them.
I am using dynabeads in the procedure. I haven't been heating the medium before I use it, however, I found out earlier this week that I was supposed to. Do you think this could be the reason they are dying?
Also, how do you change medium for cells?
What other techniques can you use??