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Isolating fetal mouse hepatocytes = possible?

Isolating fetal mouse hepatocytes = possible? - Cell Biology and Cell Culture

Isolating fetal mouse hepatocytes = possible? - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 05-26-2009, 08:28 PM
Pipette Filler
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Default Isolating fetal mouse hepatocytes = possible?



Hi all,
I'm hoping to isolate hepatocytes from C57BL/6J and A/J fetal mice at day 19 of gestation. So far the protocols I've come across involve perfusion of the liver, but I don't think I'd be able to do this with such small mice... Is there a way to isolate these cells WITHOUT having to perfuse the liver? Is perfusion an absolutely necessary step? Any help/suggestions would be greatly appreciated!
- L
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