Isolating fetal mouse hepatocytes = possible?
I'm hoping to isolate hepatocytes from C57BL/6J and A/J fetal mice at day 19 of gestation. So far the protocols I've come across involve perfusion of the liver, but I don't think I'd be able to do this with such small mice... Is there a way to isolate these cells WITHOUT having to perfuse the liver? Is perfusion an absolutely necessary step? Any help/suggestions would be greatly appreciated!
|All times are GMT. The time now is 07:34 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved