Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Cell Biology and Cell Culture (http://www.molecularstation.com/forum/cell-biology-cell-culture/)
-   -   Isolating fetal mouse hepatocytes = possible? (http://www.molecularstation.com/forum/cell-biology-cell-culture/66371-isolating-fetal-mouse-hepatocytes-%3D-possible.html)

Lauren 05-26-2009 08:28 PM

Isolating fetal mouse hepatocytes = possible?
 
Hi all,
I'm hoping to isolate hepatocytes from C57BL/6J and A/J fetal mice at day 19 of gestation. So far the protocols I've come across involve perfusion of the liver, but I don't think I'd be able to do this with such small mice... Is there a way to isolate these cells WITHOUT having to perfuse the liver? Is perfusion an absolutely necessary step? Any help/suggestions would be greatly appreciated!
- L


All times are GMT. The time now is 11:32 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09469 seconds with 11 queries