| | Re: heat inactivated serum
Since serum is a blood product, it contains complement,
which can lead to complement-mediated cell lysis. To eliminate
this risk, serum can be heat-inactivated. Heat inactivation
destroys the complement, but can also destroy heatlabile
growth factors, vitamins, amino acids, and hormones
present in serum. Damage to these from extended heating
or from temperatures greater than 56C will decrease cell
growth. Heating oftentimes can cause the proteins and
lipids concentrated at the bottom to appear as white globules.
Constant swirling during the heat inactivation process
will help solve this problem.