Serum free media - components that can be kept together

[sonyah]

Hi,

We are just starting to use serum free media. I am wondering if their are any components of the media that can be stored together (i.e. ITS and linolelic acid); or that definately should not be stored together; so that I do not have to add each component seperately each time I make up the media.

Thanks, Sonya.

[jsantagate]

Hi Sonya,

I do not know how you can store these products whether they are together or not, but I am curious to know if you are using Serum Replacement Grade BSA in your Serum free media?

[sonyah]

hi,

we are using tissue culture grade BSA, not sure if this is the same or not.

[jsantagate]

Hi Sonya,

They are similar, however the Serum Replacement Grade BSA that my company provides is specifically for use in serum-free and serum reduced cell culture applications. This BSA is highly a purified, lyophilized powder. It is formulated to optimize the products lipid profile for growth promotion, this lipid-enriched BSA is processed to provide a minimum of 98% albumin and to be essentially free of IgG. A supplementation rate ranging from .5 to 2.5 mg/mL has been effective in the production of of recombinant proteins, virus particles and monoclonal antibodies in cultured cells.
Try a free sample to see if out serum replacement grade BSA will help you with your serum free cell culture.