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Problem Transfecting Primary Cells

Problem Transfecting Primary Cells - Cell Biology and Cell Culture

Problem Transfecting Primary Cells - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 11-30-2006, 09:25 PM
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Question Problem Transfecting Primary Cells



Hey everyone,

I have a big problem transfecting primary cells with FuGene and Lipofectamine.

Do you guys have a better protocol or idea of how to transfect these primary cells?

mine are liver cells.

Thanks
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Old 12-04-2006, 10:46 PM
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Default Re: Problem Transfecting Primary Cells

Hey Molecule,

yes this is a big problem for most including our lab. We tried transfecting with FuGene which doesn't require a media change. Some people try electroporation.
I would try a few techniques and see which is healthiest for your cells (ie take a look at them in the microscope and see which one is better).

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