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A549 stimulation nitric oxide

A549 stimulation nitric oxide - Cell Biology and Cell Culture

A549 stimulation nitric oxide - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 03-28-2009, 01:05 PM
Pipette Filler
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Default A549 stimulation nitric oxide



Dear members,

I have problems with stimulating A549 cells to produce measurable amounts of nitric oxide. I tried different combinations of cytokines, also LPS 1microg/mL+IFN-gamma 100ng/mL+TNF-alpha 100ng/mL+IL-1beta 100ng/mL without any success.
I use the griess assay to measure nitrite after 18h of stimulation.
Has anyone experience or advice concerning that issue?

Thanks a lot for help in advance.
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