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#1
03-28-2009, 01:05 PM
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 A549 stimulation nitric oxide Dear members, I have problems with stimulating A549 cells to produce measurable amounts of nitric oxide. I tried different combinations of cytokines, also LPS 1microg/mL+IFN-gamma 100ng/mL+TNF-alpha 100ng/mL+IL-1beta 100ng/mL without any success. I use the griess assay to measure nitrite after 18h of stimulation. Has anyone experience or advice concerning that issue? Thanks a lot for help in advance.  |
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| a549 , nitric , oxide , stimulation |
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