A549 stimulation nitric oxide
I have problems with stimulating A549 cells to produce measurable amounts of nitric oxide. I tried different combinations of cytokines, also LPS 1microg/mL+IFN-gamma 100ng/mL+TNF-alpha 100ng/mL+IL-1beta 100ng/mL without any success.
I use the griess assay to measure nitrite after 18h of stimulation.
Has anyone experience or advice concerning that issue?
Thanks a lot for help in advance.
|All times are GMT. The time now is 11:03 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2013, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved