Starving / Pre-treatment Cells for Signalling Experiments

molecule2005 · #1 (**permalink**) 11-25-2006, 01:22 AM

Hey guys,

great to see the site forum back up.

how do you guys starve / pre-treat cells for signalling experiments? I want to treat my cells with insulin after the starvation step.

thanks

moleculardude · #2 (**permalink**) 12-04-2006, 09:45 PM

Hey there Molecule,

usually I wash the cells twice with MEM (just MEM no FBS in it!), you can also use PBS although I like MEM.

Then you simply add 1mL of MEM to each 6 well dish (volume depends on the type of dish you use) and keep the cells in that media for 1-4 hours or sometimes even overnite. That is called starving the cells.

I don't recommend more than 4-8 hours for cells, especially primary cells. You can optimize the approach to your cells.

Then after starvation you can treat cells with signalling factors.

cheers!

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Starving / Pre-treatment Cells for Signalling Experiments

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