Hi everyone. My company (SiMPore, Inc.) is developing a novel membrane filter technology that offers excellent precision in separation which we believe will be valuable to the drug development application. Our porous nanocrystalline silicon membrane can be fabricated between 5 and 25 nm thick with porosity between 5-10%. Our preliminary permeability studies show significantly greater permeability and lower electrical resistance compared to conventional transwell insert membranes. We believe these characteristics could be extended to PAMPA and Caco-2 assays.
Below are a few questions that we would value your input on.
How often is PAMPA assays used vs. Caco-2?
What specific applications potentially benefit from PAMPA vs. Caco-2?
What are the specific benefits of PAMPA vs. Caco-2?
What is limiting broader use of the PAMPA assay?
What improvements are needed to make the assay more useable or effective?
Would incorporation of a porous nanocrystalline silicon membrane help improve the PAMPA assay's usefulness? Would it help address any performance issues with the Caco-2 assay?
Thanks in advance for your feedback.