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PAMPA vs. Caco-2 Assay for Drug Permeability

PAMPA vs. Caco-2 Assay for Drug Permeability - Cell Biology and Cell Culture

PAMPA vs. Caco-2 Assay for Drug Permeability - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 02-19-2009, 05:33 PM
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Default PAMPA vs. Caco-2 Assay for Drug Permeability



Hi everyone. My company (SiMPore, Inc.) is developing a novel membrane filter technology that offers excellent precision in separation which we believe will be valuable to the drug development application. Our porous nanocrystalline silicon membrane can be fabricated between 5 and 25 nm thick with porosity between 5-10%. Our preliminary permeability studies show significantly greater permeability and lower electrical resistance compared to conventional transwell insert membranes. We believe these characteristics could be extended to PAMPA and Caco-2 assays.

Below are a few questions that we would value your input on.

How often is PAMPA assays used vs. Caco-2?

What specific applications potentially benefit from PAMPA vs. Caco-2?

What are the specific benefits of PAMPA vs. Caco-2?

What is limiting broader use of the PAMPA assay?

What improvements are needed to make the assay more useable or effective?

Would incorporation of a porous nanocrystalline silicon membrane help improve the PAMPA assay's usefulness? Would it help address any performance issues with the Caco-2 assay?

Thanks in advance for your feedback.
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assay , caco2 , drug , pampa , permeability


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