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Elevated concentrations of ascorbate and normoxia suppresstestosterone production in cultured guinea pig Leydig cells

Elevated concentrations of ascorbate and normoxia suppresstestosterone production in cultured guinea pig Leydig cells - Cell Biology and Cell Culture

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Old 11-29-2008, 04:04 PM
tocopherol
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Default Elevated concentrations of ascorbate and normoxia suppresstestosterone production in cultured guinea pig Leydig cells



Elevated concentrations of ascorbate and normoxia suppress
testosterone
production in cultured guinea pig Leydig cells

by Mark A. Kukucka and Hara P. Misra
Department of Biomedical Sciences
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute & State University, Blacksburg 24061


ABSTRACT

In recent years, several metabolic roles have been proposed for
vitamin C.
Recent information suggests a strong causal relationship between high
endogenous levels of ascorbic acid and changes in normal reproductive
biology. Using highly enriched populations of guinea pig Leydig cells,
we
have found that elevated levels (50 to 500 microM) of ascorbate
significantly (P < 0.01) depressed testosterone production in a
dose-dependent manner while low levels (0 to 10 microM) were without
effect.
Leydig cells incubated under hypoxic (3% oxygen) culture conditions
produced
significantly (P < 0.01) more testosterone than similar cells cultured
under
normoxic (19% oxygen) conditions. The results of this study suggest
that
high concentrations of ascorbate and normoxic culture conditions
suppress
testosterone production in isolated Leydig cells. Thus, it would seem
that
there exists a delicate balance between normal metabolic requirements
for
vitamin C and excessive ascorbate levels that might alter normal
gonadal
reproductive events.


CITATION
Kukucka, Mark A. and Hara P. Misra. 1994. Elevated concentrations of
ascorbate and normoxia suppress testosterone production in cultured
guinea
pig Leydig cells. Reprod Toxicol 8(4):333-9.


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ascorbate , cells , concentrations , cultured , elevated , guinea , leydig , normoxia , pig , production , suppresstestosterone


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