Hi - I'm trying to find information about how to maintain isolated human NK cells in culture. I dont need to have them proliferate, but I'm trying to ascertain the correct conditions to keep them viable for functional assays over the course of about a week. The critical thing seems to be the amount of IL2 used, but I would like to use an approximation of physiological conditions, and not activate them so much with IL2 that they are activated regardless of what antigens they encounter. I've been using RPMI, 10% FBS, and 1% pen/strep and 50U/ml IL2...does that seem correct? There seems to be too much cell death. Thanks so much!