erithrocytes lysis to obtain lymphocytes for inmunofluorescence

bocha_25 · #1 (**permalink**) 10-02-2006, 11:08 PM

Hello! dOES ANYBODY KNOWS HOW TO LYSE ERYTHROCYTES WITHOUT BREAKING THE LYMPOHOCYTES IN ORDER TO USE THOSE FOR INMUNOFLUORESCENCE. iT´S JUST THAT I CULTIVATE WHOLE BLOOD, AND AFTER THAT i NEED TO FIXATE THE CELLS JUST WITH METHANOL. iN THIS WAY i CANNOT TAKE OUT THE RED BLOOD CELLS SO, i THOUGH OF BREAKING THE RED BLOOS CELLS, WASHE THE PELLET, AND OBATIN JUST LYMPHOS, BUT THE AMOUNT OF THIS ONES I OBTAIN AFTER USING A SOLUCIÓN WITH AMONIUM, IS NOT ENOUEGH TY.

admin · #2 (**permalink**) 10-05-2006, 01:42 AM

Hey Bocha,
I will look this information up for you.

admin · #3 (**permalink**) 10-06-2006, 06:32 PM

Hey Bocha,
please confirm these but there is:

Oxygen radical-induced erythrocyte hemolysis by neutrophils
Neutrophils lyse erythrocytes by oxygen radicals

Erythrocyte Lysis Solution is used to lyse red blood cells specifically

The chemical component of ELS is ammonium chloride which serves to lyse red blood cells exclusively.

If this is helpful, please post here back thanks

admin · #4 (**permalink**) 10-10-2006, 07:14 PM

Hope this helped you,
please post back here

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Member Login

erithrocytes lysis to obtain lymphocytes for inmunofluorescence

Cell Biology and Cell Culture

Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.