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Missing GST protein

Missing GST protein - Cell Biology and Cell Culture

Missing GST protein - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 10-10-2008, 06:19 PM
Pipette Filler
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Default Missing GST protein



I am trying to express a GST-tagged protein, and I used a Centricon-70 Plus to concentrate my sample after FPLC because I couldn't see a band on my SDS. After concentration, the concentrate and the flow through had approximately the same A280 and I still can't see anything on SDS (staining with Coomassie).
Any ideas?
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