Cell Passage Number for Cell Culture

kiki06 · #1 (**permalink**) 09-05-2006, 07:01 PM

Dear fellows,
what is the maximum cell passage number during subculturing? I have heard this is 30, however what is the optimal cell passage number?

Does this also depend on the cell line?

thanks

Rhombus · #2 (**permalink**) 09-06-2006, 10:14 AM

Every cell line is different. Established cell lines generally can be passaged more than primary cells. I have passaged J774 Mouse Macrophages over 200 times without problems. The most important thing to check is that the receptor/cytokines/enzyme/protein that you are interested in is STABLE over that period of time.
We have used endothelial primaries and found that certain prostaglandins change almost instantly, within 1 or 2 passages. Make sure to have banked early passage cells so if there are changes, you have cells to re initiate.

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Cell Passage Number for Cell Culture

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