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Transformation Analysis

Transformation Analysis - Cell Biology and Cell Culture

Transformation Analysis - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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  #1  
Old 06-12-2008, 12:59 PM
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Default Transformation Analysis



Hi

after transformation of cells by chemical or radiation means, what is the best and simple way to confirm the transformation of animal cells?



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aftab
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Old 07-03-2008, 02:45 AM
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Default Re: Transformation Analysis

Are you talking about genetic mutation? I wonder if there is some way to stain for thymine dimers.....

Last edited by ethidiumbromide; 07-03-2008 at 03:16 AM.
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Old 07-11-2008, 11:53 AM
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Default Re: Transformation Analysis

yes i am talking about genetic mutation that make the normal cells to cancerous cells. so how we can confirm that cells are transformed....

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aftab
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Old 06-18-2012, 07:02 AM
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Default Re: Transformation Analysis

looking
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Old 08-01-2012, 01:41 PM
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Default Re: Transformation Analysis

I am guessing a change in cell surface markers.
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Old 08-01-2012, 03:37 PM
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Default Re: Transformation Analysis

The cheapest way can be to quantify the growth rate. Compare the time it take to achieve confluence for the same amount of initial cells or compare cell cycle by FACS. cancer cells have a tendency to skip the Go phase. they should have greater proportion in Gs and G2
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