Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Cell Biology and Cell Culture (http://www.molecularstation.com/forum/cell-biology-cell-culture/)
-   -   Transformation Analysis (http://www.molecularstation.com/forum/cell-biology-cell-culture/3449-transformation-analysis.html)

aftabac 06-12-2008 12:59 PM

Transformation Analysis
 
Hi

after transformation of cells by chemical or radiation means, what is the best and simple way to confirm the transformation of animal cells?



regards
aftab

ethidiumbromide 07-03-2008 02:45 AM

Re: Transformation Analysis
 
Are you talking about genetic mutation? I wonder if there is some way to stain for thymine dimers.....

aftabac 07-11-2008 11:53 AM

Re: Transformation Analysis
 
yes i am talking about genetic mutation that make the normal cells to cancerous cells. so how we can confirm that cells are transformed....

regards
aftab

hairongxu88 06-18-2012 07:02 AM

Re: Transformation Analysis
 
looking

Richard Cowart 08-01-2012 01:41 PM

Re: Transformation Analysis
 
I am guessing a change in cell surface markers.

boum13 08-01-2012 03:37 PM

Re: Transformation Analysis
 
The cheapest way can be to quantify the growth rate. Compare the time it take to achieve confluence for the same amount of initial cells or compare cell cycle by FACS. cancer cells have a tendency to skip the Go phase. they should have greater proportion in Gs and G2


All times are GMT. The time now is 02:05 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09239 seconds with 11 queries