How Do Cells Adhere to Culture Flasks?

[joey20]

Hello,
i am not sure why i cant find a good answer to this question but how do cells attach to the surface of tissue culture flasks?

thanks

[danfive]

1st most mammalian cells are adherent, so the extracellular membrane will contain hydrophobic glycoproteins & mucins in general and integrins, cadherins, selectins and other cell-adhesion-molecules (CAMs) in particular.
2nd some culture flasks will be protein coated (protein tends to stick to most non-natural substances) and will likely have ligand properties that will attract the adherent cells.
3rd other flasks will be coated with inert chemical monolayers like PEG, tri-Ethylene Glycol which can act as substrate/ligands for various proteins in the ECM, most likely glycoproteins

[danfive]

Didn't find too many sources, this was most useful to me --->http://www.rsc.org/ej/CS/2000/a705397e.pdf "A surface chemistry approach to studying cell adhesion"