How To Transport Live Cells Lines?

[dnamolecule]

Hello all, i have a cell line transportation question

How should one transport live cell lines? Is this done cold or warm?

thanks

[marrie11]

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[rdittmar]

Typically cell lines are sent on dry ice.

[marcusho]

I've sent live cells many times via mail, one time all the way to Tunisia (took 1.5 weeks), and the cells were always alive when they arrived (sometimes barely)

-Put a small amount ~2e6 cells into a 15ml conical and fill to the top with media. Have it incubate in 37'

-for more fragile cells that are adherent, you can put it in a T-25 flask and fill it to the top with media. Although the media will get the lil filter on the top of the cap and prevent air flow, I found that cells can still survive the trip.

-you can also add extra factors depending on what's usually in the cell media if you are worried about viability. (extra FBS, antoxidants, etc)

-When packaging, keep the cells VERY stable and upright. Use alot of newspaper, foam peanuts, or those plastic airbags. whenever you receive shipments it's good for this reason to save packing materials if you intend to send out live cell samples later

[marcusho]

-and yes it must be warm as close to 37' as possible. theres no good way to keep cells heated but keep the cells in an airtight styrofoam box to minimize temperature changes and they should stay at room temperature hopefully.