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Fetal Calf Serum FCS or Fetal Bovine Serum FBS?

Fetal Calf Serum FCS or Fetal Bovine Serum FBS? - Cell Biology and Cell Culture

Fetal Calf Serum FCS or Fetal Bovine Serum FBS? - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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  #1  
Old 03-05-2008, 07:37 AM
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Default Fetal Calf Serum FCS or Fetal Bovine Serum FBS?



Hiya, i am confused what is the difference between Fetal Calf Serum FCS and Fetal Bovine Serum FBS? which is better of the 2?
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Old 03-05-2008, 05:19 PM
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Default Re: Fetal Calf Serum FCS or Fetal Bovine Serum FBS?

well
FCS and FBS are the same and there is no difference in these. but mostly Fetal Calf serum brand is used. and this serum is collected from pregnant cows who are brought to slaughter, so after slaughtering of cow, fetus is taking out, that is more then 3 months old and needle is injected into its heart and blood is collected aspeptically and then serum is collected after centrifugation process and then finally it is heat treated and saled.

regards
aftab
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Old 07-27-2008, 07:42 PM
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Default Re: Fetal Calf Serum FCS or Fetal Bovine Serum FBS?

Does FCS contains more growth factors or other molecules that stimulate cell division than FBS? I have cultured chicken fibroblast with DMEM+10% FBS for 3 days and there seems no obvious increase of cell number.
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Old 07-28-2008, 06:46 PM
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Default Re: Fetal Calf Serum FCS or Fetal Bovine Serum FBS?

In undergrad and grad school we used the fetal bovine and fetal calf interchangeable but the purchased product was always called Fetal Bovine Serum. Depending on who you talk to, some will say that the statement "fetal calf serum" is incorrect since the developing bovine is technically not a calf till birth. You might say fetal human or fetal canine, but you would never say fetal infant or fetal puppie. Thus a "fetal calf" is actually a "fetal bovine" and serum from such would be fetal bovine serum as aftabac mentioned.

Definition of "calf" = at birth
[Only registered users see links. ]

Definition of "fetus" = prior to birth
[Only registered users see links. ]

"Calf" would then be most appropriate used as "New Born Calf" Serum. New Born can mean different things depending on how long they let the calf age/ween before harvesting the serum. So, you will often see great differences in brands and lots of new born calf serum. The developing fetus will have a different profile of hormone and growth factors than a new born so certain cells are grown in fetal vs calf serum.

Regardless, age of the fetus (or calf) and the time of the year that the blood is collected will affect how well it peforms in a paticular culture experiment because of the diet of the mother of the fetus or nursing calf or if the calf has started grazing. Therefore, cetain labs will do extensive lot testing prior to buying serum.

Hope that helps.
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Old 09-18-2008, 12:18 AM
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Default Re: Fetal Calf Serum FCS or Fetal Bovine Serum FBS?

Hi everyone
I accidentally left my FBS (new bottle, have not yet opened)at room temperature overnight. can it still be used. can i heat inactivate it?
Thanks y'all
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Old 04-12-2009, 07:24 PM
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Default Re: Fetal Calf Serum FCS or Fetal Bovine Serum FBS?

Hello,
as far as i know u cannot heat inactivate fcs as the proteins will denature. If u have not opened it yet...it might be ok to go ahead and use it...
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Old 04-16-2009, 12:31 AM
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Default Re: Fetal Calf Serum FCS or Fetal Bovine Serum FBS?

Quote:
Originally Posted by raptorliang View Post
Does FCS contains more growth factors or other molecules that stimulate cell division than FBS? I have cultured chicken fibroblast with DMEM+10% FBS for 3 days and there seems no obvious increase of cell number.
I am working with AML12 cells in DMEM/F12 and DMEM and found that using undenatured serum makes a big difference. We used denatured FBS and our cells did not grow. Switched to undenatured and they took off within hours. Denaturing may kill off the growth factors.

Last edited by Nepenthe; 04-16-2009 at 12:33 AM.
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Old 12-01-2010, 01:37 AM
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Default Re: Fetal Calf Serum FCS or Fetal Bovine Serum FBS?

FCS stands for "Football Championship Subdivision"(the old Division 1-AA), which uses a playoff to determine the national championship, hence the name "Championship Subdivision."

FBS stands for "Football Bowl Subdivision" (the old Division 1-A), which uses the post-season bowl games (specifically the Bowl Championship Series (the BCS) to determine a national championship) hence the "Bowl Subdivision".

The NCAA changed the names a few years ago, because they felt that the 1-A and 1-AA designations implied that the FCS was inferior.

Both are technically subdivisions of Division 1, not separate divisions, and teams from one can play teams from the other.

The primary differences are the higher number of scholarship players FBS schools are required to have on their rosters, a seating capacity requirement (20,000 min., I believe) for FBS stadiums and attendance requirements (something on the order of 16000 fans/home game for 2 consecutive years). Thus, schools which wish to make the transition from FCS to FBS must make substantial financial investments to upgrade stadiums and facilities and to add scholarships.

Teams which have successfully made the jump from FCS to FBS include Boise St, Idaho, Connecticut, South Florida, Central Florida, Toledo, Miami (Oh.), Northern Illinois, Troy and Middle Tennessee. Western Kentucky is in its first full year since making the jump, with Texas St., UT-San Antonio and South Alabama scheduled to make the move in 2012 and UMass-Amherst and Villanova exploring the possibility of moving up in 2013,

I hope that helps.
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Old 12-06-2010, 09:06 PM
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Default Re: Fetal Calf Serum FCS or Fetal Bovine Serum FBS?

Quote:
Originally Posted by Diana N View Post
Hello,
as far as i know u cannot heat inactivate fcs as the proteins will denature. If u have not opened it yet...it might be ok to go ahead and use it...
Inactivating FBS is an ought to if you are working with cells. Serum contains complement proteins and antibodies. The complement proteins are highly reactive to cells from a different species, in other words, the complement will lyse any cell that isn't, in this case, bovine. In the other hand, antibodies may block some cell receptors which may harm or alter your cultured cells.
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Old 01-27-2011, 05:07 PM
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Default Re: Fetal Calf Serum FCS or Fetal Bovine Serum FBS?

Just as a little addition to this... does anyone know any references that suggest how much BSA is in FBS? I understand that BSA is a rather large component of FBS!

Help would be well appreciated!

Thanks!
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