| | Re: Fetal Calf Serum FCS or Fetal Bovine Serum FBS?
Has anyone ever had problems with FBS and mold? We have been having issues with our cultures developing mold...I thought it was the incubator not having been cleaned out. We have not had mold contamination for a week and a half now, after cleaning the incubators thoroughly, but last week I noticed a partial 50ml tube of FBS had large debris in it, so I cultured it carefully in a Petri dish and today there is a mold bloom in it. However, my cultures that I used the FBS in when I first thawed it are still clean. I did not use the open tube of FBS and just made fresh media with a new tube. Now that tube has large bits of debris floating in it, so I am in turn thawing a different tube.
Strangely, my coworker hasn't had the same contamination since the first week of the month, and we are using FBS aliquots from the same bottles.