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Cell Adhesion Problem After Transfection

Cell Adhesion Problem After Transfection - Cell Biology and Cell Culture

Cell Adhesion Problem After Transfection - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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  #1  
Old 03-05-2008, 07:35 AM
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Question Cell Adhesion Problem After Transfection



Hello guys, I have another problem....


This time its cell detachement after transfection. Can someone help?:
  1. I am seeding HEK293 (260,000 cells/well in the dish) in 24-well plates.
  2. I transfect 24h post seeding. I am transfecting using lipofectamine.
  3. Cells grow fine. I wash them with 1mL HBSS (Gibco) and incubate them for 10 minutes before transfection. Cells looks fine, but seem a bit stressed after the incubation.
  4. I add my transfection mixture, and right after transfection the cells start to detach from the bottom of the plates.
I now have changed the media to Optimem, got new and better plates, and nothing helps!


thanks!
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Old 04-09-2008, 02:40 PM
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Default Re: Cell Adhesion Problem After Transfection

Hello there

it may be you are transfecting with too much DNA / transfection agent or your cells may not be healthy.

Have you tried this again or optimized your transfection?
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