Hello guys, I have another problem....
This time its cell detachement after transfection. Can someone help?:
- I am seeding HEK293 (260,000 cells/well in the dish) in 24-well plates.
- I transfect 24h post seeding. I am transfecting using lipofectamine.
- Cells grow fine. I wash them with 1mL HBSS (Gibco) and incubate them for 10 minutes before transfection. Cells looks fine, but seem a bit stressed after the incubation.
- I add my transfection mixture, and right after transfection the cells start to detach from the bottom of the plates.
I now have changed the media to Optimem, got new and better plates, and nothing helps!