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Comparison of Cell Lysis Methods

Comparison of Cell Lysis Methods - Cell Biology and Cell Culture

Comparison of Cell Lysis Methods - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 01-10-2008, 03:05 PM
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Exclamation Comparison of Cell Lysis Methods



Hiya dudes has any1 compared the different cell lysis methods?

what should you use when?

for example is homogenization a tissue specific method, or can you use it for cells too???

Also freeze thawing? when do you use it??


Cell Lysis Methods I found:
  • Sonication method
  • French press method
  • Freeze Thaw method
  • Passive lysis method
  • Homogenization
Is there a convention when to use these?
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Old 01-10-2008, 10:01 PM
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Default Re: Comparison of Cell Lysis Methods

You can homogenize tissue and cells and bone, etc
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Old 01-10-2008, 10:02 PM
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Default Re: Comparison of Cell Lysis Methods

I've used freeze thawing for lysis of gram-positive bacteria for DNA extraction.
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Old 01-08-2009, 03:55 PM
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Default Re: Comparison of Cell Lysis Methods

Has anyone had experience with Algae cells and lysing those? Most lab equipment is pretty expensive and we're on a budget. Can't get my hands on a french press, the closest thing to a rotor stator homogenizer is a handheld immersion blender but I don't know if it will create the shear force needed to break open the cells,, what do you guys think? We're trying to extract the oil inside the algae cells.
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Old 01-14-2009, 11:36 PM
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Smile Re: Comparison of Cell Lysis Methods

Quote:
Originally Posted by CHEGNerd View Post
Has anyone had experience with Algae cells and lysing those? Most lab equipment is pretty expensive and we're on a budget. Can't get my hands on a french press, the closest thing to a rotor stator homogenizer is a handheld immersion blender but I don't know if it will create the shear force needed to break open the cells,, what do you guys think? We're trying to extract the oil inside the algae cells.
I worked with picophytoplankton once upon a time and used CTAB extraction---got great genomic data out of it.
For oil, this is just a guess, freezing and hand homogenizing with mortar and pestle ---should give you OK data ---and using a fancy motor hand homogenizer would give excellent data (I've used this on Arabidopsis and Medicago--so I could test for oxalate).

Here's a look at the fancy homogenizer ---> [Only registered users see links. ]

Try borrowing one if you can, they are pricey.
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Old 03-02-2009, 10:10 PM
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Default Re: Comparison of Cell Lysis Methods

Dear friends,

Does anyone know which way is the best way to disrupt and homogenize whole animal tissue for good RNA purification, is it rotor-stator homogenizer? or ultrasonicator? thanks a bunch!

bbsun
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