Comparison of Cell Lysis Methods

[tonyz06]

Hiya dudes has any1 compared the different cell lysis methods?

what should you use when?

for example is homogenization a tissue specific method, or can you use it for cells too???

Also freeze thawing? when do you use it??


Cell Lysis Methods I found:

• Sonication method
• French press method
• Freeze Thaw method
• Passive lysis method
• Homogenization

Is there a convention when to use these?

[danfive]

You can homogenize tissue and cells and bone, etc

[danfive]

I've used freeze thawing for lysis of gram-positive bacteria for DNA extraction.

[CHEGNerd]

Has anyone had experience with Algae cells and lysing those? Most lab equipment is pretty expensive and we're on a budget. Can't get my hands on a french press, the closest thing to a rotor stator homogenizer is a handheld immersion blender but I don't know if it will create the shear force needed to break open the cells,, what do you guys think? We're trying to extract the oil inside the algae cells.

[danfive]

Originally Posted by CHEGNerd

Has anyone had experience with Algae cells and lysing those? Most lab equipment is pretty expensive and we're on a budget. Can't get my hands on a french press, the closest thing to a rotor stator homogenizer is a handheld immersion blender but I don't know if it will create the shear force needed to break open the cells,, what do you guys think? We're trying to extract the oil inside the algae cells.

I worked with picophytoplankton once upon a time and used CTAB extraction---got great genomic data out of it.
For oil, this is just a guess, freezing and hand homogenizing with mortar and pestle ---should give you OK data ---and using a fancy motor hand homogenizer would give excellent data (I've used this on Arabidopsis and Medicago--so I could test for oxalate).

Here's a look at the fancy homogenizer ---> [Only registered users see links. ]

Try borrowing one if you can, they are pricey.

[bbsun]

Dear friends,

Does anyone know which way is the best way to disrupt and homogenize whole animal tissue for good RNA purification, is it rotor-stator homogenizer? or ultrasonicator? thanks a bunch!

bbsun