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Function of EDTA in Trypsin

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Function of EDTA in Trypsin

Cell Biology and Cell Culture

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#1 (permalink)

01-10-2008, 02:01 PM

postdock

Pipette Filler

Join Date: Feb 2006

Posts: 15

Function of EDTA in Trypsin

Hi, I am curious what is the function of EDTA in trypsin for cell subculturing?
cheers

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#2 (permalink)

01-22-2008, 12:26 PM

binxue

Pipette Filler

Join Date: May 2007

Location: China

Posts: 6

Re: Function of EDTA in Trypsin

to delete Ca2+, Mg2+, for these metal ions can reduce the function of trypsin.

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Function of EDTA in Trypsin

Cell Biology and Cell Culture

Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.