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-   -   Proliferation assays in Co-culture system (http://www.molecularstation.com/forum/cell-biology-cell-culture/27527-proliferation-assays-co-culture-system.html)

Padma Channavajhala 08-29-2008 04:53 PM

Proliferation assays in Co-culture system
 
Hi,

I am trying to assess the proliferation of Endothelial cells which are grown in the same dish as a stromal layer. (Can't use inserts to grow endothelial system since for our purposes they need to be together this way)... Now I want to know if anybody had done
a) proliferation assays of endothelial cells in this set up..
b) in our assays we grow endothelial cells for 3 days after adding them to stromal layer..
c) If I consider Brdu assay: how long i need to pulse these cells with Brdu? Followed by BrDu can I separate endothelial cells with let's say anti-CD31 antibody conjugate to Dynal beads?
d) after I separate out these cells with Dynal beads, are these activated? Can I grow these separated cells?

can somebody give me their opinion or suggestion or if they have used this sort of experiment please share your experience..

Thanks






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