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permeabilize living cells

permeabilize living cells - Cell Biology and Cell Culture

permeabilize living cells - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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  #1  
Old 05-21-2008, 09:27 PM
assa sittner
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Default permeabilize living cells



Hi there,
I am looking for a reagent that would allow me to weakly permeabilize, or
just weaken cell membranes in a living cell culture, without killing them,
in order to facilitate microinjection.
does anyone know such a reagent (most likely a mild surfactant)?
cheers,
t
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  #2  
Old 05-23-2008, 01:50 PM
Nick Theodorakis
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Default permeabilize living cells

On May 21, 5:27*pm, "assa sittner" <[Only registered users see links. ].fr> wrote:

I've seen a couple of references where people have used low
concentrations of mild detergents, but cells usually hate it when you
make then leaky.

If you are injecting regular cultured cells with a glass
microcapillary there should be no reason to make the membrane weaker;
the capillary will go right through.

Nick

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  #3  
Old 05-27-2008, 10:47 PM
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Default permeabilize living cells

i have read research that pulsed electric fileds at certain frequencies
transiently cause cancer cells to become porous
thus enabling increased delivery of chemotherapeutic agents into the cell

the pulse by itself as used on living tissue isn't designed to kill the
cells it just induces them to open channels through which substrates will
flow

search "electroporation cell membrane" at
[Only registered users see links. ]

on the internet there is a video of a news clip of the principle being used
to treat a man
for melanoma on his foot

it's not a chemical reagent but it does make them permeable

as far as chemicals go you are on the right track with surfactants - i
also have come across "saponins" which will do the same thing

search "saponin permeable cell membrane" at
[Only registered users see links. ]

there are some books that talk in detail about plant saponins in this regard
on google books

i have read plant saponins are used to make nanoparticles for drug delivery
of chemo drugs too

perhaps they facilitate penetration of the drug conjugate through the cell
membrane


----- Original Message -----
From: "assa sittner" <[Only registered users see links. ].fr>
Newsgroups: bionet.cellbiol
To: <[Only registered users see links. ].indiana.edu>
Sent: Wednesday, May 21, 2008 2:27 PM
Subject: permeabilize living cells





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