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Immunofluorescence with two rabbit antibodies together.

Immunofluorescence with two rabbit antibodies together. - Cell Biology and Cell Culture

Immunofluorescence with two rabbit antibodies together. - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 01-08-2008, 03:31 AM
Morgan Xu
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Default Immunofluorescence with two rabbit antibodies together.




Dear all,

Has anybody ever tried immunofluorescence with two primary antibodies
both developed from rabbits?

One of the antibodies is conjugated with Alexa 488 directly. I am
planning to apply the non-conjugated primary and the corresponding
secondary first. Then fix it with paraformaldehyde. And last applying
the conjugated primary.

The concern is how well the secondary antibody will be held by
fixation. Also how well the Alexa dye will stand the paraformaldehyde
fixation.

Any suggestion will be appreciated. Thanks.

Morgan
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