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Q: fast cell counting methods

Q: fast cell counting methods - Cell Biology and Cell Culture

Q: fast cell counting methods - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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  #1  
Old 07-08-2007, 04:05 PM
Porpyrin
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Default Q: fast cell counting methods



Does any body have a suggestion for easier and faster cell counting
methods than using haemacytometer? Counting cells is a tidious job
especially when there are lots of samples to count. It seems using
coulter, a particle counter, is one substitute. However a coulter is
not cheap and sometimes cells block the tube in the coulter. I
experienced often. Is there any other good way to count cancer cells?

Thanks!

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  #2  
Old 07-09-2007, 05:38 AM
Eric
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Default Q: fast cell counting methods

Porpyrin wrote:

Have you looked into flow cytometry to see if it's compatible with your
protocol?

[Only registered users see links. ]

[Only registered users see links. ]

Eric
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  #3  
Old 07-09-2007, 02:20 PM
Porpyrin
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Default Q: fast cell counting methods

On Jul 9, 12:38 am, Eric <[Only registered users see links. ]> wrote:

Thanks Eric for your comment.

But, I never thought about using FACS and can not access FACS.

Porphyrin

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  #4  
Old 07-10-2007, 02:04 AM
Bob
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Default Q: fast cell counting methods

On Mon, 09 Jul 2007 14:20:02 -0000, Porpyrin <[Only registered users see links. ]>
wrote:


If you don't want low-tech counting and don't want high-tech counting,
maybe you could consider an alternative to counting.

How about measuring something else, such as amount of DNA or protein?
These can be related to cell number -- with assumptions and caveats.
It is up to you to decide whether they are appropriate for your needs.
If so, then look for something that is fairly simply to do with
numerous samples.

bob



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