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S phase cell marker for immunofluorescence

S phase cell marker for immunofluorescence - Cell Biology and Cell Culture

S phase cell marker for immunofluorescence - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 08-17-2006, 01:59 PM
Dr Engelbert Buxbaum
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Default S phase cell marker for immunofluorescence



Collynn Woeller wrote:



S phase is characterised by DNA doubling, so any DNA stain can be used
as long as you can quantify it. This includes the fluorescent nuclear
dyes like propidium iodide or Hoechst and the non-fluorescent Feulgen
staining. Quantitation by fluorescence is easy (FACS, image processing
microscopy), Feulgen can be quantified by a microscope-linked
photometer, specialised equipment that is probably rare nowadays. The
technique however was extensively used 20, 30 years ago for life cycle
investigations (determining which are the haploid and diploid forms).
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