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[Cell-biology] RNAse ( BLOCKERING CHEMICALS) - Cell Biology and Cell Culture

[Cell-biology] RNAse ( BLOCKERING CHEMICALS) - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.

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Old 03-09-2006, 08:05 PM
Jayakumar, R
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Default [Cell-biology] RNAse ( BLOCKERING CHEMICALS)

Dear Damodar
Generally, you take care of RNAses while you do the RNA preparation.
All solutions should be treated with DEPC and all surfaces and pipettes
etc should be RNAse free. Store the freshly prepared RNA in RNAse free
microfuge tubes and store them immediately in -80C. the RNA can be
dissolved in DEPC treated water (which is free of RNAses).
DEPC is probably the most widely used RNA degrading chemical in the
market. But you might have to degrade the DEPC by autoclaving after
treating solutions with it. You can find the complete protocols for
this in "Current protocols in molecular biology".
Best of luck


From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of Damodar
Sent: Thursday, March 09, 2006 7:27 AM
To: [Only registered users see links. ]
Subject: [Cell-biology] RNAse ( BLOCKERING CHEMICALS)

Dear sir/madam,

I Damodar working as scientist at Reametrix India PVt Ltd. Sir I have
been working on RNA storage focused on the chemicals which degrade
RNAses . sir can you send me some details about it.

Awaiting for ur reply,

Thanks and regards,



Reametrix India PVt Ltd

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