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[Cell-biology] Soft agar cloning of Tumor cells

[Cell-biology] Soft agar cloning of Tumor cells - Cell Biology and Cell Culture

[Cell-biology] Soft agar cloning of Tumor cells - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 01-12-2006, 10:00 PM
Jayakumar, R
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Default [Cell-biology] Soft agar cloning of Tumor cells



how about using an some enzymes like agarase to dissolve the agar. I am not sure what effect it would have on the cells? Does agarase dissolve agar or just agarose?

jai


-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu]On Behalf Of Ken Garson
Sent: Thursday, January 12, 2006 10:24 AM
To: [Only registered users see links. ]
Subject: [Cell-biology] Soft agar cloning of Tumor cells



Hi,

I am screening primary tumor cells for their ability to grow in soft agar.
I have recovered adherent cell lines by sucking out the colonies with a
pipette and then mechanically breaking up the agar/cell plug to release
the tumor cells. The mechanical disruption is not very efficient. Is
there a method for recovering colonies from soft agar and generating a
nice single cell suspension of viable cells?

Thanks!

Ken

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