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Soft agar cloning of Tumor cells

Soft agar cloning of Tumor cells - Cell Biology and Cell Culture

Soft agar cloning of Tumor cells - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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  #1  
Old 01-12-2006, 03:23 PM
Ken Garson
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Default Soft agar cloning of Tumor cells




Hi,

I am screening primary tumor cells for their ability to grow in soft agar.
I have recovered adherent cell lines by sucking out the colonies with a
pipette and then mechanically breaking up the agar/cell plug to release
the tumor cells. The mechanical disruption is not very efficient. Is
there a method for recovering colonies from soft agar and generating a
nice single cell suspension of viable cells?

Thanks!

Ken

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  #2  
Old 01-17-2006, 10:13 AM
Dr Engelbert Buxbaum
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Default Soft agar cloning of Tumor cells

Ken Garson wrote:


Have you tried to place the agar pieces into cell culture dishes with
medium and waiting for the cells to grow out (that is, without
disruption of the agar piece)?
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