Soft agar cloning of Tumor cells

Hi,

I am screening primary tumor cells for their ability to grow in soft agar.
I have recovered adherent cell lines by sucking out the colonies with a
pipette and then mechanically breaking up the agar/cell plug to release
the tumor cells. The mechanical disruption is not very efficient. Is
there a method for recovering colonies from soft agar and generating a
nice single cell suspension of viable cells?

Thanks!

Ken

Ken Garson wrote:


Have you tried to place the agar pieces into cell culture dishes with
medium and waiting for the cells to grow out (that is, without
disruption of the agar piece)?