Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture
Register Search Today's Posts Mark Forums Read

Cell Biology and Cell Culture Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


Concentric circles in 293 cells

Concentric circles in 293 cells - Cell Biology and Cell Culture

Concentric circles in 293 cells - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-05-2006, 06:22 PM
D.
Guest
 
Posts: n/a
Default Concentric circles in 293 cells



I am culturing 293 cells (from monkey kidney) in DMEM in a 10cm dish.
I've probably gone through about 15 passages using identical protocols,
but noticed something strange this morning. Two confluent plates from
the last split had a pattern of concentric circles (bullseye pattern).
Looked at under a microscope the lines of the circles appeared as very
low density cells in an otherwise confluent plate.

Any idea why these appeared?

Thanks,
..dan

Reply With Quote
  #2  
Old 01-06-2006, 02:41 AM
Nick Theodorakis
Guest
 
Posts: n/a
Default Concentric circles in 293 cells

On 5 Jan 2006 10:22:57 -0800, "D." <[Only registered users see links. ]> wrote:


My guess is that it's a defect of the plastic dish.

Nick

--
Nick Theodorakis
[Only registered users see links. ]
contact form:
[Only registered users see links. ]
Reply With Quote
  #3  
Old 01-06-2006, 01:54 PM
D.
Guest
 
Posts: n/a
Default Concentric circles in 293 cells

Could be. I've been using the same bag of about 20 dishes for the past
few weeks. Both dishes split from one 293 dish had the same pattern.
Another two dishes split from a C2C12 line and put on dishes right
afterwards from the same pack did not show the pattern. I looked at
cells split from the patterned 293 cells yesterday and the same pattern
does not seem to be developing.

Thanks,
..d


Nick Theodorakis wrote:

Reply With Quote
Reply

Tags
293 , cells , circles , concentric


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
mouse hepatocytes isolation amicia Cell Biology and Cell Culture 39 05-11-2012 11:52 AM
Characteristics of cancerous cells aftabac Cell Biology and Cell Culture 2 08-26-2011 11:40 PM
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
How to isolate adult from circulation? peterish Stem Cell Forum 0 05-19-2008 10:02 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 12:37 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.16156 seconds with 16 queries