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About attaching PC12

About attaching PC12 - Cell Biology and Cell Culture

About attaching PC12 - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 11-14-2005, 02:09 AM
nutellina
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Default About attaching PC12



I'm having same problem of many of the people here in the newsletters
about PC12. I'm always using polylysine and/or collagen for attaching
PC12 to the bottom of plastic wells, dishes and flasks. But now that
I'm trying to have a "carpet" of PC12 attached very well to the bottom
of a glass slide, I cannot find a solution, it seems that polylysine
and collagen are not working properly, the cells are not attached
and/or they detach easily even if I wait for days. I also bought
treated glass slides from NUNC but the problem remain. Where is the
mistake? Can anybody help me?

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