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red fluorescent DNA staining

red fluorescent DNA staining - Cell Biology and Cell Culture

red fluorescent DNA staining - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 09-15-2005, 01:58 AM
Huseyin Sumer
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Default red fluorescent DNA staining



Dear Jos,

I came across your question on GFP and using a vital red fluorescent dye
for DNA staining on the web. Did you get anywhere with all this? I am
also looking into using an alternate to H33342 and GFP, your help will
be greatly appreciated.

kind regards

Huseyin



__________________________________________________ _______________

Dear reader,

Currently I am examining living cells with Green Fluorescent Protein
and I want to combine this with a red fluorescent dye to visualize DNA
in vital cells using confocal laser scanning microscopy. I cannot use
Hoechst or other dyes with UV excitation, since cells will be damaged
and since our laser cannot excite Hoechst. Does anyone know of a vital
red fluorescent DNA dye with absorption spectrum around 567 nm and
emission above 600 nm? I already tried several dyes from Molecular
Probes (Syto-17 and Syto-64) but they appear to stain no nuclear DNA in
living eukaryotic cells.

Thank you very much for your reply.
Jos Broers




--
Huseyin Sumer Ph.D.
NH&MRC Peter Doherty Biomedical Research Fellow
Centre For Reproduction and Development
Monash Institute of Medical Research

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