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Breast cancer cell lines: rapid characterization and identification of an apocrine cell line.

Breast cancer cell lines: rapid characterization and identification of an apocrine cell line. - Cell Biology and Cell Culture

Breast cancer cell lines: rapid characterization and identification of an apocrine cell line. - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 09-08-2005, 07:41 AM
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Default Breast cancer cell lines: rapid characterization and identification of an apocrine cell line.



Molecular characterization of breast cancer cell lines by a low density
microarray.

by Françoise de Longueville (*), Marc Lacroix (*), Anna-Maria Barbuto,
Vincent Bertholet, Dominique Gallo, Denis Larsimont, Laurence Marcq,
Nathalie Zammatteo, Sophie Boffe, Guy Leclercq and Jose Remacle
(*): equal contributions
Institut Jules Bordet Institute, Bruxelles (Brussels) and Eppendorf
Array Technologies (EAT), Namur, Belgique (Belgium)
in International Journal of Oncology (2005) 27, 881-892

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We designed a low density microarray carrying 132 DNA capture sequences
highly specific for genes known to be differentially expressed among
breast tumors and breast cancer cell (BCC) lines or associated with
specific tumor properties (hormone sensitivity, cell cycle alteration,
proteolysis, adhesion, etc). We analyzed gene expression in 11 BCC
lines among which 6 had already been extensively studied (BT-474,
Hs578T, MCF-7, MDA-MB-231, MDA-MB-453, T-47D) and 5 were still poorly
characterized (Evsa-T, IBEP-1, IBEP-2, IBEP-3, KPL-1). Some data
obtained were verified or extended by real-time polymerase chain
reaction, Northern-blotting, Western blotting, immunohistochemistry and
cell growth studies. Clustering analysis of the low-density microarray
data allowed the sorting of BCC lines into two classes and supported a
major discriminatory role for ER-alpha, confirming data from previous
studies. A few genes that are highly and specifically expressed in one
cell line were identified such as MGB1 / SCGB2A2 (mammaglobin 1 /
secretoglobin family 2A, member 2) in Evsa-T cells, and PIP / GCDFP15
(prolactin-inducible protein / gross cystic disease fluid protein-15 )
in MDA-MB-453 BCC, suggesting an apocrine origin for these latter
cells. Two BCC lines (IBEP-1 and IBEP-3) that had been previously
characterized as ER-alpha-negative, were classified by the low density
microarray among ER-alpha-positive lines (MCF-7, T-47D, IBEP-2, BT-474,
KPL-1) and were indeed confirmed as receptor-positive (at both mRNA and
protein levels) and hormone-responsive cells. In conclusion, our
results support the use of a low density microarray approach in cases
where the cost and exhaustiveness of high density microarrays may
constitute a drawback; for instance, in obtaining a rapid phenotype
evaluation in cell populations freshly isolated from breast tumors.

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apocrine , breast , cancer , cell , characterization , identification , line , lines , rapid


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