I use the Cell Death Detection ELISA from Roche for quantification of
apoptosis. The problem is that the cells under investigation show
considerable differences in proliferation, so when the assay is done
the cell number will not be the same.
Therefore, I want to normalize the assay results with a suitable
parameter like cell number or protein content (since the cells in the
wells used for the ELISA are lysed, wells seeded with the same number
of cells in parallel). However, the very small volume of each assay
sample makes it difficult to count cells accurately or determine the
Has anyone ever done any normalization for the Cell Death Detection
ELISA (Roche) and if yes, how did you do it?