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Cell Culture Tips

Cell Culture Tips - Cell Biology and Cell Culture

Cell Culture Tips - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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  #1  
Old 08-08-2006, 06:42 AM
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Arrow Cell Culture Tips



Does anyone have any Cell Culture Tips?

ie sterile culture tips, working with cell lines, etc?

thanks in advance for any tips....


Kiki
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Old 08-23-2006, 09:34 AM
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Default Re: Cell Culture Tips

well.. only tip is to be extra careful when u work.. media preparations must be in autoclaved bottles etc.. aseptic technique is important.. use autoclaved or sterile tips... when transfering media becareful not to touch the neck of the bottle etc...
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Old 08-23-2006, 05:00 PM
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Default Re: Cell Culture Tips

Some good tips... what about use of ethanol? spray everything down with ethanol or is it not very useful?
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Old 11-15-2006, 11:50 AM
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Default Re: Cell Culture Tips

Haik,

I have read in a Biochemistry Laboratory book by Rodney Boyer and it is mentioned that using acetone to speed up the drying of glasswares is not advisable as it will leave the non-volatile organic residues on the surface and might affect the bacterial growth. So, I am not sure if that aplies the same to ethanol.

However, ethanol is used to sterilise the laminar flow and work area prior to usage. The ethanol used is usually mixed with water.
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Old 11-17-2006, 12:57 AM
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Default Re: Cell Culture Tips

Thank you!

very nice tip Hsip!

Also never touch your culture plastics to anything! If you do, get new one.
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