Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Cell Biology and Cell Culture (http://www.molecularstation.com/forum/cell-biology-cell-culture/)
-   -   Cell Culture Tips (http://www.molecularstation.com/forum/cell-biology-cell-culture/262-cell-culture-tips.html)

kiki06 08-08-2006 06:42 AM

Cell Culture Tips
 
Does anyone have any Cell Culture Tips?

ie sterile culture tips, working with cell lines, etc?

thanks in advance for any tips....


Kiki

MicroKiller 08-23-2006 09:34 AM

Re: Cell Culture Tips
 
well.. only tip is to be extra careful when u work.. media preparations must be in autoclaved bottles etc.. aseptic technique is important.. use autoclaved or sterile tips... when transfering media becareful not to touch the neck of the bottle etc...

oBWhat 08-23-2006 05:00 PM

Re: Cell Culture Tips
 
Some good tips... what about use of ethanol? spray everything down with ethanol or is it not very useful?

hsip_15 11-15-2006 11:50 AM

Re: Cell Culture Tips
 
Haik,

I have read in a Biochemistry Laboratory book by Rodney Boyer and it is mentioned that using acetone to speed up the drying of glasswares is not advisable as it will leave the non-volatile organic residues on the surface and might affect the bacterial growth. So, I am not sure if that aplies the same to ethanol.

However, ethanol is used to sterilise the laminar flow and work area prior to usage. The ethanol used is usually mixed with water.

darkwng 11-17-2006 12:57 AM

Re: Cell Culture Tips
 
Thank you!

very nice tip Hsip!

Also never touch your culture plastics to anything! If you do, get new one.


All times are GMT. The time now is 12:14 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09552 seconds with 11 queries