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11-12-2007, 02:53 AM
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 Marsupial chromosomes Hi everyone, I'm doing some cultures of kangaroo lymphocytes (72h/37ºC) and after arresting them with 0.5 microgram/ml colchicine (1h), shocikng them with o.o56 KCl, and fixing them in methanol:acetic acid I don't see a single metaphase. Not even bad looking metaphases, as if the colchicine exposure were too long. Any clue? Thanks!        |
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