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how to identify truly apoptosis

how to identify truly apoptosis - Cell Biology and Cell Culture

how to identify truly apoptosis - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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  #1  
Old 12-13-2003, 01:38 AM
jaymone
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Default how to identify truly apoptosis



Not true that any drug can induce apoptosis. Many drugs kill cells without
inducing apoptosis. The only way to identify apoptosis-inducing drugs is to
expose cells to the drug and look for markers of apoptosis, such as caspase
activation, DNA laddering, chromatin condensation, etc.

Jay Mone

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  #2  
Old 12-15-2003, 01:00 PM
Tom Anderson
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Default how to identify truly apoptosis

On 13 Dec 2003, jaymone wrote:


But that won't distinguish between drugs which have an effect which the
cell responds to by apoptosing (eg etoposide, which causes DNA damage) and
drugs which trigger apoptosis directly, by acting on the apoptotic
machinery. I'm not certain, but i think that's what the OP was getting at.

I have no advice myself, except to consult a pharmacologist, who ought to
know about this sort of stuff.

tom

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Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL

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  #3  
Old 12-16-2003, 11:31 AM
Nath
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Default how to identify truly apoptosis

I have trained as a pharmacologist, and have done work on apoptosis as part
of my year in industry during my pharmacology degree. What you seem to be
asking is how do you differentiate between drugs that induce the apoptotic
machinery and drugs that damage the cell and the cell responds by going into
apoptosis.

This simple answer is "not easy". You would need to do extensive lab work on
each drug to identify it's mechanism of action, by systematically inhibiting
enzymes in the apoptotic pathways and/or have cell lines that lack a certain
enzyme in the apoptotic pathways. Needless to say, finding the mechanism of
action for drug is not a trivial matter, and requires extensive work (ofetn
years). Also, apoptotic pathways are somewhat redundent, hence, if you
inhibit an enzyme in the apoptotic pathway that you think your drug is
inducing, the inhibited enzyme can be bypassed by one of the alternative
apoptotic routes.

It depends on what your final goal is, it is often enough to look for the
signs of apoptosis.....reply with further details of what you are trying to
do.

Nathan


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