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endothelial cells on collagen type I

endothelial cells on collagen type I - Cell Biology and Cell Culture

endothelial cells on collagen type I - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 10-07-2003, 02:51 PM
Dana L.
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Default endothelial cells on collagen type I



Hello everybody,

I have been trying to grow aortic bovine endothelial cells on collagen
type I originated in ratí»s tails.
When the layer of collagen is thin (about a millimeter) the cells
proliferate like on a culture dish. As the thickness of the collagen
increase, fewer cells last, and when the thickness of the collagen is
about 4 millimeters, non survive.

What could be the reason for this phenomena?

Thanx in advance
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