How Do you Harvest Your Cells?

admin · #1 (**permalink**) 11-09-2007, 02:52 PM

also

Why do different cell lines have different protocols for cell detachment?

proatpcr · #2 (**permalink**) 11-09-2007, 02:54 PM

I LOVE passive lysis buffer with a rotator! easy on the hands

kskaare · #3 (**permalink**) 11-29-2007, 02:53 PM

Trypsin, but for "picky" cultures i use non-enzymatic cell dissociation buffer.

Laleh Kamalian · #4 (**permalink**) 01-25-2008, 12:28 PM

If your cells are in suspension, like mine are, you only need to spin them down in order to harvest them. However I think the reason for different protocoles for different cells is the fact that inter-cellular attachments are different among different cell lines, so is the adherency to the flask. As I said some cells even grow in suspension and these are not only blood cell lines. Maybe it has something to do with the memberane carachtristics. I have also dealth in past with cells which are semi-adherent to the flask. To harvest them I only needed to wash them down with PBS a few times.

View Poll Results: How Do You Harvest Your Cells?
Trypsinization	5	62.50%
Scraping Cells	1	12.50%
Freeze Thawing	0	0%
Other	2	25.00%
Voters: 8. You may not vote on this poll

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How Do you Harvest Your Cells?

Cell Biology and Cell Culture

Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.