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cell activity

Cell Biology and Cell Culture

Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.



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  #1 (permalink)  
Old 11-03-2007, 07:08 AM
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Smile cell activity

hello all!

does older Agrobacterium glycerol stocks(cells with 50% glycerol) at -80C can loose their activity?
If so then how to regain their activity?


thanks and regards,

varsha.
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  #2 (permalink)  
Old 11-04-2007, 11:59 AM
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Default Re: cell activity

Hello there Varsha!


Did you freeze thaw them at all?
or anyone else?
cheers
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Old 11-05-2007, 04:55 AM
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Default Re: cell activity

Hello all!,

No,I do not freez thaw them,

actually long storage at -80c can destroy the activity?


thanks and regards,

varsha
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