Mdck

kskaare · #1 10-24-2007, 06:56 PM

For some reason, my MDCK cells keep crashing out. Any tips? Using EMEM, 10% FBS, pen/strep and adding glutamax (glutamine supplement). Seeding density is 1e6/T-75.

Thanks

mutant11 · #2 12-08-2011, 01:21 AM

I grow them in DMEM (low glucose). They grow very quickly so I do splits at 1:20-1:30.

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Mdck - Cell Biology and Cell Culture

Mdck - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.