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For some reason, my MDCK cells keep crashing out. Any tips? Using EMEM, 10% FBS, pen/strep and adding glutamax (glutamine supplement). Seeding density is 1e6/T-75.
I grow them in DMEM (low glucose). They grow very quickly so I do splits at 1:20-1:30.
|Thread||Thread Starter||Forum||Replies||Last Post|
|MDCK cell||sholva||Cell Biology and Cell Culture||0||07-10-2007 09:26 AM|
|cellules mdck||sholva||Forum de Biologie Moleculaire||0||07-06-2007 08:36 AM|