Originally Posted by vinnnervinayak
I have tried ths protocol to make activated sodium orthovanadate. However when i do add HCL to the 100-200mM solution to adjust ph to 10 it does turn yellow.However i tried boiling it for several minutes (20-30) and it still did not turn colourless.any suggestion in this regard would be really helpful !
How about making Water at pH 10.
Then using the pH 10 water to dissolve the orthovanadate.
Once dissolved insert the bottle or tube into boiling water bath for 10 min or until the yellow color disappears (tends to happen immediately with fresh ortho).
Just so you know yellow solution is actually decavanadate, the wrong configuration to inhibit lysosomal enzymes. So you need to get it clear (orthovanadate configuration) by boiling it.
If you cannot get the ortho to be clear by changing the steps for preparation, then you need to buy fresher orthovanadate.